Journal: PLoS ONE

Article Title: How Do Human Cells React to the Absence of Mitochondrial DNA?

doi: 10.1371/journal.pone.0005713

Figure Lengend Snippet: (A) Sub-organellar localization of Elac2 by western-blot analysis on mitochondria isolated from HeLa cells. Specificity of antibody reactivity was proved by the CRM obtained using an in vitro translated (i.v.) Elac2. A CRM was present in mitochondria (M), in the mitochondrial matrix (Ma) and membranes (Mb), and barely detectable in the nucleus (N). ETHE1 antibody was used as a mitochondrial matrix control protein. (B) Proteinase K protection assay by western-blot analysis on freshly isolated mitochondria from HeLa cells. Lane 1, cell lysate; lane 2, intact mitochondria; lane 3, mitochondria treated with 100 µg/ml of proteinase K; lane 4, mitochondria treated with 0.5% of Triton-X100. (C) Mitochondrial in vivo import of HA-tagged human Elac2 N-terminal polypeptide expressed in Cos-7 cells. Lane 1: marker 46 kDa (MW). lane 2: immunoprecipitation of total radiolabeled proteins. lane 3: immunoprecipitation of total radiolabeled proteins after the addition of valinomycin that determines the elimination of the mitochondrial ΔΨ: as a consequence, mitochondrial import is abolished. lane 4: immunoprecipitation of in vitro translation product (i.v.). Arrows indicate the Elac2 precursor (Elac2) and the mature form (mElac2). (D) Confocal immunofluorescence on Cos7 cells. The green fluorescence corresponds to Elac2HA-specific immunoreactivity. The red fluorescence corresponds to immunoreactivity specific to mtSSB. The two immunofluorescence patterns overlap, as shown by confocal merge. Magnification: 40×.

Article Snippet: An affinity purified polyclonal antibody against ELAC2 was from ProteinTech, while the polyclonal antibody against Ethe1 was raised in rabbit by Neosystem .

Techniques: Western Blot, Isolation, In Vitro, Control, In Vivo, Marker, Immunoprecipitation, Immunofluorescence, Fluorescence

Journal: Biomolecules

Article Title: Comparative Analysis of Testicular Transcriptional and Translational Landscapes in Yak and Cattle–Yak: Implications for Hybrid Male Sterility

doi: 10.3390/biom15081080

Figure Lengend Snippet: Comparison of transcription and translation levels between yak and cattle–yak testes. Correlation analysis of translational and transcriptional expression levels between cattle–yak ( A ) and yak ( B ). ( C ) Comparison and classification chart of translational and transcriptional differences. GO enrichment analysis of genes in the Homodirection ( D ) and Translation ( E ) groups. ( F ) PPI network diagram of genes involved in spermatogenesis. ( G ) RNA-seq and Ribo-seq IGV visualization of PIWIL1. ( H ) PPI network diagram of meiosis-related genes, including those involved in meiotic chromosome segregation, chromosome organization involved in meiotic cell cycle, meiotic cell cycle process, and meiotic cell cycle. ( I ) WB detection of the expression levels of MEI1 and FTSJ1 (* represents p < 0.05). Original figures can be found in .

Article Snippet: The rabbit polyclonal antibodies used included MEI1 (1:500, bs-6387R, Bioss, Beijing, China) and FTSJ1 (1:1500, 11620-1-AP, Proteintech, Wuhan, China), and the mouse polyclonal antibody used was β-actin (1:4000, 66009-1-AP, Proteintech).

Techniques: Comparison, Expressing, RNA Sequencing